FastQCFastQC Report
Wed 17 Jul 2019
T3_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT3_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20907830
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA1073740.5135587959152146Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT616960.29508562103288577Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610960.2922158827577993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG38700.050.793944
ATACGAC51300.038.3176886
AATACGA61500.032.548425
TACGACT63850.030.5597577
CTAGTTG99600.029.8565448
ACCTTAG116150.025.9122319
TACTCTA118450.025.7137574
ACGACTC81250.024.015678
CGACTCA82900.023.1033469
ATAATAC88850.023.0180153
ACTCTAG134450.022.9215095
TCTAGTT137700.022.6409957
CTCTAGT143850.022.024036
CACCTTA147850.020.453938
GTACTCT159850.020.2274283
TACTACC113850.018.6573165
TAGTTGT167000.018.2808829
GAATAAT116300.017.9586661
TTACTAC119900.017.4756774
TGTACTC202900.016.2569182
Produced by FastQC (version 0.11.5)