FastQCFastQC Report
Wed 17 Jul 2019
T3_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT3_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20907830
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2534861.2123974606642582TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC1227050.5868853917407977TruSeq Adapter, Index 6 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT1120630.5359858005350149Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG537880.2572624705672468Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC340900.089.9494259
TTCCCTA368250.083.034258
ACACTCT411600.075.532861
CACTCTT444050.069.972442
ACTCTTT456150.068.226693
TTTCCCT543800.056.9307637
CTTTCCC547200.056.6969386
TCTTTCC572450.054.5237775
CTCTTTC590850.052.912264
TAATACG37400.051.9799544
ATACGAC57300.033.800796
AATACGA64300.031.1292155
TACGACT66250.029.2337257
CTAGTTG100350.027.0484078
ACGACTC86250.022.7887928
CGACTCA86750.022.4084639
TACACGA282700.022.32508910-14
TAGTTGT127800.021.9710859
ATAATAC90550.021.9532263
CTACACG297300.021.10280610-14
Produced by FastQC (version 0.11.5)