FastQCFastQC Report
Wed 17 Jul 2019
T2_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT2_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16668850
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA601060.36058876287206376Illumina Single End PCR Primer 1 (97% over 34bp)
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG319470.19165689294702395No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT285740.1714215437777651Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG234010.14038760922319177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG39850.055.4707874
ACCTTAG131850.045.325149
ATACGAC51700.042.477196
CACCTTA149500.040.31138
AATACGA55850.039.8372735
CTAGTTG94800.038.431278
TACGACT61850.035.2730377
ACTCTAG118300.031.2238835
TACTCTA118150.030.6540974
TCTAGTT126200.029.8959167
CGACTCA72850.029.74949
ACGACTC72700.029.513758
CTCTAGT128200.029.261476
ATAATAC76700.029.0114543
TAGTTGT141100.026.1777089
GAATAAT96050.025.6445481
GTACTCT156500.023.9734083
TTTCCAC266800.022.7777524
TTCCACC271000.022.5841585
TGTACTC195200.019.5550882
Produced by FastQC (version 0.11.5)