FastQCFastQC Report
Wed 17 Jul 2019
T2_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT2_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16668850
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1107380.6643409713327555TruSeq Adapter, Index 5 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT627040.3761747211115344Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC588380.35298175938952TruSeq Adapter, Index 5 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG229870.13790393458456943Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA182120.10925768724297116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC240300.067.893099
TTCCCTA252550.064.6854558
ACACTCT273200.060.4992751
TAATACG40200.057.315154
CACTCTT295200.056.4107252
ACTCTTT310700.053.341063
CTTTCCC391100.042.581056
TTTCCCT394100.042.2012187
TCTTTCC397100.042.0285845
ATACGAC55700.041.4933436
CTCTTTC405400.041.009624
AATACGA58950.039.4502645
CTAGTTG101050.036.6223568
TACGACT64150.035.6902777
ACTCTAG121600.030.8485895
TAGTTGT121900.030.4174659
TCTAGTT126750.030.0488037
CTCTAGT131900.028.8215776
ATAATAC82050.028.5292933
CGACTCA81750.027.5661459
Produced by FastQC (version 0.11.5)