FastQCFastQC Report
Wed 17 Jul 2019
T1_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT1_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16125093
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA680860.4222363244664697Illumina Single End PCR Primer 1 (97% over 34bp)
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG431460.26757054982566614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343160.21281117572469194No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT337390.20923290178853543Illumina Single End PCR Primer 1 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA211150.13094498121654244No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC209420.12987211918715755No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG189150.11730164905095432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG45900.058.8269044
ACCTTAG151550.051.025939
CTAGTTG111100.046.0139548
ATACGAC58900.045.2308126
CACCTTA172100.044.8915028
AATACGA61850.043.656515
TACGACT66800.039.9884577
ACTCTAG132950.038.994345
TCTAGTT136650.038.3582657
CTCTAGT140300.037.3612756
TACTCTA152600.033.925934
ATAATAC81150.033.897683
ACGACTC79600.033.287418
TAGTTGT156200.033.188999
CGACTCA82800.031.8268269
GAATAAT93000.030.200341
TTTCCAC268200.029.1828824
GTACTCT184050.028.7180773
TTCCACC278100.028.1957935
TGTACTC209800.025.2641772
Produced by FastQC (version 0.11.5)