FastQCFastQC Report
Wed 17 Jul 2019
T1_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT1_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16125093
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC764240.4739445533740488TruSeq Adapter, Index 4 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT711450.441206757691258Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC451370.2799177654355234TruSeq Adapter, Index 4 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG315320.19554615902060224Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA257730.15983163631986497No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG224240.13906276385506738No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA196180.12166131382932178No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG187070.11601173400984416No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC181710.11268772217313723TruSeq Adapter, Index 4 (98% over 50bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC175570.108879992196014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC240150.078.099119
TTCCCTA270750.069.378778
ACACTCT284700.066.65761
TAATACG45100.064.9785164
CACTCTT304550.062.5772552
ACTCTTT312900.060.8603483
TTTCCCT392550.048.7507137
CTCTTTC407400.047.189154
TCTTTCC405500.047.124115
CTTTCCC407900.047.023216
ATACGAC64800.044.8888136
AATACGA67150.043.6396755
CTAGTTG119200.043.307588
TACGACT73100.040.2834747
ACTCTAG137950.038.20525
TAGTTGT136150.038.0746429
CTCTAGT144050.036.68726
TCTAGTT144650.036.5838857
ATAATAC86150.034.695363
CGACTCA88050.032.707829
Produced by FastQC (version 0.11.5)