FastQCFastQC Report
Wed 17 Jul 2019
C3_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC3_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16178102
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA801530.49544130702105843Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG782090.4834250643246037No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT384320.23755567865748403Illumina Single End PCR Primer 1 (97% over 37bp)
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG228170.14103632181327574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG30450.040.6732564
ACCTTAG107000.038.356249
ATACGAC36300.034.5138136
CACCTTA127200.032.5482038
CTAGTTG75300.031.6504088
AATACGA45500.027.852815
TACGACT47750.026.9910167
TACTCTA95000.025.694724
ACTCTAG102050.024.4841065
TCTAGTT103550.023.7802057
CTCTAGT110450.022.881886
CGACTCA61050.020.2855939
ACGACTC61450.020.270788
ATAATAC63650.020.1388663
GTACTCT133750.019.4368933
TAGTTGT127600.019.2983599
TTTCCAC238250.017.4385624
TTCCACC242700.017.1484875
TACTACC90450.016.7177755
GAATAAT85000.016.0140781
Produced by FastQC (version 0.11.5)