FastQCFastQC Report
Wed 17 Jul 2019
C3_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC3_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16178102
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1624531.0041536392835204TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC899520.5560108348927457TruSeq Adapter, Index 3 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT848400.5244125670613278Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG349640.21611929508170985Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCTA285400.076.413218
TCCCTAC288550.075.728749
ACACTCT320350.068.981781
CACTCTT339350.065.372912
ACTCTTT349500.063.432773
TAATACG28600.053.6240354
TTTCCCT426150.052.0537837
CTTTCCC428800.051.8841676
TCTTTCC429100.051.6299255
CTCTTTC447050.049.8805274
ATACGAC43350.035.543146
CTAGTTG75450.033.4943058
AATACGA48000.032.255
TACGACT52350.030.2572277
TAGTTGT100450.025.5882979
ACTCTAG102250.025.067975
TCTAGTT108050.024.5880857
ATAATAC67650.023.636193
CTCTAGT113100.023.108686
CGACTCA68600.021.8304339
Produced by FastQC (version 0.11.5)