FastQCFastQC Report
Wed 17 Jul 2019
C2_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC2_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19463703
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA746750.3836628620977211Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG446160.2292266790137519No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT389120.19992084753862097Illumina Single End PCR Primer 1 (97% over 37bp)
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG330360.1697313198829637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG39300.052.216554
ACCTTAG147150.041.6893549
ATACGAC51950.038.5308346
CACCTTA170100.036.1492588
CTAGTTG102100.035.895328
AATACGA58200.035.630785
TACGACT64800.031.667157
ACTCTAG132800.028.3569495
TCTAGTT138100.027.7889827
TACTCTA141450.026.4192394
CTCTAGT144550.026.1511256
ACGACTC76300.025.8566258
CGACTCA78900.024.8220549
ATAATAC85850.024.4092453
TAGTTGT153600.024.1413659
GTACTCT180800.021.30873
TTTCCAC301650.020.5998134
GAATAAT104600.020.5869181
TTCCACC306000.020.3775675
TACTACC122550.018.6252375
Produced by FastQC (version 0.11.5)