FastQCFastQC Report
Wed 17 Jul 2019
C2_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC2_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19463703
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1806910.9283485264854279TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1151510.5916191795569425TruSeq Adapter, Index 2 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT780070.40078190671117414Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG346740.1781469846719301Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC299350.069.845099
TTCCCTA320550.065.383028
ACACTCT355650.059.94551
CACTCTT365850.058.470392
TAATACG38600.055.7730984
ACTCTTT384650.055.630763
TCTTTCC478950.044.6471525
TTTCCCT494000.043.358937
CTCTTTC496050.043.3125954
CTTTCCC503200.042.466816
ATACGAC57450.037.5973786
AATACGA60550.036.0293125
CTAGTTG107300.035.8981938
TACGACT68850.031.5806857
TAGTTGT132100.029.540319
ACTCTAG138350.028.9349755
TCTAGTT145100.027.6876017
CTCTAGT150000.026.6396246
ATAATAC89450.025.0413723
CGACTCA86850.024.1235779
Produced by FastQC (version 0.11.5)