FastQCFastQC Report
Wed 17 Jul 2019
C1_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC1_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16170615
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA707710.4376518765674652Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT383550.23718949464816272Illumina Single End PCR Primer 1 (97% over 37bp)
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG297530.18399423893278022No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290070.1793809326361428No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC161930.10013843010918261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG42050.050.171544
ACCTTAG122000.045.620579
ATACGAC52050.041.638356
CACCTTA141200.039.7744038
CTAGTTG102000.039.03638
AATACGA56800.037.649915
TACGACT61400.034.475617
ACTCTAG121800.033.1052255
TACTCTA120900.032.75614
TCTAGTT127850.031.9312047
CTCTAGT135000.030.0277236
CGACTCA75200.028.1494889
ACGACTC75350.027.9025128
TAGTTGT147600.026.9761419
GTACTCT154550.026.2344363
ATAATAC82950.026.045773
GAATAAT93700.023.7518961
TTTCCAC246050.023.0307034
TACTACC108500.022.8288765
TTCCACC260400.021.844495
Produced by FastQC (version 0.11.5)