FastQCFastQC Report
Wed 17 Jul 2019
C1_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC1_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16170615
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1006800.6226108283451186TruSeq Adapter, Index 1 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT730460.4517206055552Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC473620.2928892933261969TruSeq Adapter, Index 1 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG340060.21029503206897202Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA191550.11845560604837849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC251300.079.074499
TTCCCTA274950.072.194298
ACACTCT296850.067.766741
CACTCTT316150.063.8359452
ACTCTTT327650.061.374633
TAATACG44850.058.437414
CTTTCCC414500.048.89776
TTTCCCT411700.048.861347
TCTTTCC414350.048.7245645
CTCTTTC421400.048.167334
ATACGAC62500.041.9330026
CTAGTTG106600.039.10588
AATACGA67800.038.973885
TACGACT69050.037.8497287
TAGTTGT123250.034.0566149
ACTCTAG123600.034.019795
TCTAGTT129850.032.381037
ATAATAC83750.032.1640323
CTCTAGT136250.031.2309786
CGACTCA83300.030.8562419
Produced by FastQC (version 0.11.5)